Publications
2025
Sirinukunwattana, K., Klein, C., Clarke, P. F. A., Marcou, G., Meyer, L., Collongues, N., de Sèze, J., Hellwig, P., Patte-Mensah, C., El Khoury, Y., Mensah-Nyagan, A.G. 'Assessment of the concomitant action of XBD173 and interferon β in a mouse model of multiple sclerosis using infrared marker bands' (2025), Spectrochim. Acta A, 327, 125390.
Juna Rauch; Katharina Kurscheidt; Kai-Wei Shen; Andreea Andrei; Noel Daum; Yavuz Öztürk; Frederic Melin; Gunhild Layer; Petra Hellwig; Fevzi Daldal; Hans-Georg Koch 'The Small Membrane Protein CcoS is Involved in Cofactor Insertion into the cbb3-type Cytochrome c Oxidase' (2025) Biochim. Biophys. Acta, 1866, 149524.
2024
Mauger, M., Makarchuk, I., Molter, Y., Sansone, A., Melin, F., Chaignon, P., Schaeffer, P., Adam, P., Schünemann, V., Hellwig, P., Ferreri, C., Chatgilialoglu, C., Seemann, M. 'Towards Bacterial Resistance via the Membrane Strategy: Enzymatic, Biophysical and Biomimetic Studies of the Lipid cis-trans Isomerase of Pseudomonas aeruginosa' (2024) ChemBioChem, e202400844.
Harter, C., Melin, F., Hoeser, F., Hellwig, P., Wohlwend, D., Friedrich, T. 'Quinone chemistry in respiratory complex I involves protonation of a conserved aspartic acid residue' (2024) Febs. Lett., 598, 2856-2865.
Sun, J., Zhou, Y., Melin, F., Hellwig, P., Boudon, C., Bonnefont, A., Ruhlmann, L., Badets, V. 'Nitrous acid electroreduction on macroporous silver foam' (2024) J. Electroanal. Chem., 964, 118326.
Bouraguba, M., Schmitt, A.M., Yelisetty, V. S., Vileno, B., Melin, F., Glattard, E., Orvain, C., Lebrun, V., Raibaut, L., Illbert, M., Bechinger, B., Hellwig, P., Gaidon, C., Sour, A., Faller, P. 'Quest for a stable Cu-ligand complex with a high catalytic activity to produce reactive oxygen species' (2024) Metallomics, 16, mfae020.
Sharma, V., Hellwig, P. Pereira, M. 'Computational and experimental insights in proton and ion translocating bioenergetic systems' (2024) Front. Chem., 12, 1384385.
2023
Makarchuk, I., Gerasimova, T., Kägi, J., Wohlwend, D., Melin, F., Friedrich, T., Hellwig, P. ‘Mutating the environment of heme b595 of E. coli cytochrome bd-I oxidase shifts its redox potential by 200 mV without inactivating the enzyme' (2023) Bioelectrochemistry, 151, 108379.
Makarchuk, I., Kägi, J., Gerasimova, T., Wohlwend, D., Friedrich, T., Melin, F., Hellwig, P. ‘pH-dependent kinetics of NO release from E. coli bd-I and bd-II oxidase reveals involvement of Asp/Glu58B' (2023) Biochim. Biophys. Acta, 1864, 148952.
2022
Kägi, J., Makarchuk, I.,Wohlwend, D., Melin, F., Friedrich, T., Hellwig, P. ‘E. coli cytochrome bd-I requires Asp58 in the CydB subunit for catalytic activity' (2022) Febs. Lett., 596, 2418-2424.
Schirer, A., El Khoury, Y., Patte-Mensah, C., Klein, C., Meyer, L., Rataj-Baniowska, M., Moss, D., Mensah-Nyagan, A.-G., Lecomte, S., Hellwig, H. 'Raman and infrared microscopic study on the lipid redistribution in Alzheimer diseased murine tissue' (2022) Asian J. Phys., 31, 259-264.
Falcone, E., Ritacca, A.G., Hager, S., Schueffl, H., Vileno, B., El Khoury, Y., Hellwig, P., Kowol, C.R., Heffeter, P., Sicilia, E., Faller, P. ‘Copper-Catalyzed Glutathione Oxidation is Accelerated by the Anticancer Thiosemicarbazone Dp44mT and Further Boosted at Lower pH’ (2022) J. Am. Chem. Soc., 144, 14758-14768.
Kalout, H., Boubegtiten-Fezoua, Z., Maurel, F., Hellwig, P., Ferlay, S. ‘An accurate vibrational signature in halogen bonded molecular crystals’ (2022) Phys. Chem. Chem. Phys., 24, 15103-15109.
El Khoury, Y., Gebelin, M., de Sèze, J., Patte-Mensah, C., Marcou, G., Varnek, A.. Mensah-Nyagan, A.-G., Hellwig, P., Collongues, N. ‘Rapid Discrimination of Neuromyelitis Optica Spectrum Disorder and Multiple Sclerosis Using Machine Learning on Infrared Spectra of Sera’ (2022) Int. J. Mol. Sci., 23, 2791.
Omeis, F. Boubegtiten-Fezoua, Z., Seica, A.F.S., Bernard, R., Iqbal, M.H., Javahiraly, N., Vergauwe, R.M.A., Majjad, H., Boulmedais, F. Moss, D., Hellwig, P. ‘Plasmonic Resonant Nanoantennas Induce Changes in the Shape and the Intensity of Infrared Spectra of Phospholipids’ (2022) Molecules, 27, 62.
Schimpf, J., Oppermann, S., Gerasimova, T., Seica, A.F.S., Hellwig, P., Grishkovskaya, I., Wohlwend, D., Haselbach, D., Friedrich, T. ‘Structure of the peripheral arm of a minimalistic respiratory complex I’ (2022) Structure, 30, 80-94.e4.
2021
Makarchuk, I., Seica, A.F.S., Melin, F., Hellwig, P. ‘Probing the reaction of membrane proteins via infrared spectroscopies, plasmonics and electrochemistry’ (2021) Curr. Opin. Electrochem., 100770.
Grauel, A., Kägi, J. Rasmussen, T. Makarchuk, I. Oppermann, S. Moumbock, A., Wohlwend, D., Müller, R., Melin, F., Günther, S., Hellwig, P., Böttcher, B., and Friedrich, T., ‘Structure of Escherichia coli cytochrome bd-II type oxidase with bound aurachin D’ (2021) Nat. Commun., 12:6498.
Das, A., Jobelius, H., Schleinitz, J., Gamboa-Ramirez, S., Creste, G. Kervern, G., Raya, J., Le Breton, N., Guénet, A., Boubegtiten-Fezoua, Z., Grimaud, L., Orio, M., Rogez, G., Hellwig, P., Choua, S., Ferlay, S. Desage-El Murr, M. ‘A hybrid bioinspired catechol-alloxazine triangular nickel complex stabilizing protons and electrons’ (2021) Inorg. Chem. Front., 8, 5286-5298.
Sharma, V., Imhof, P. Hellwig, P. ‘Editorial: Computational and Experimental Insights in Redox-Coupled Proton Pumping in Proteins’ (2021) Front. Chem., 9, 762612.
Seiça, A.F.S., Iqbal, M.H., Carvalho, A., Choe, J.Y., Boulmedais, F., Hellwig, P. ‘Study of Membrane Protein Monolayers Using Surface-Enhanced Infrared Absorption Spectroscopy (SEIRAS): Critical Dependence of Nanostructured Gold Surface Morphology’ (2021) ACS sensors, 6, 2875-2882.
Nikolaev, A., Safarian, S., Thesseling, A., Wohlwend, D., Friedrich, T., Michel, H., Kusumoto, T., Sakamoto, J., Melin, F., Hellwig P. ‘Electrocatalytic evidence of the diversity of the oxygen reaction in the bacterial bd oxidase from different organisms’ (2021) Biochim. Biophys. Acta, 1862, 148436.
El Khoury, Y., Schirer, A., Patte-Mensah, C., Klein, C., Meyer, L., Rataj-Baniowska, M., Bernad, S., Moss, D., Lecomte, S., Mensah-Nyagan, AG., Hellwig, P. ‘Raman Imaging Reveals Accumulation of Hemoproteins in Plaques from Alzheimer's Diseased Tissues’ (2021) ACS Chem. Neurosci., 12, 2940-2945.
Makarchuk, I, Nikolaev, A., Thesseling, A., Dejon, L., Lamberty, D., Stief, L., Speicher, A., Friedrich, T., Hellwig, P., Nasiri, H.R., Melin, F. ‘The inhibition study of cytochrome bd oxidase using the enzyme-based electrochemical sensor’ (2021) Chem. Proc., 5, 45.
Makarchuk, I., Nikolaev, A., Thesseling, A., Dejon, L., Lamberty, D., Stief, L., Speicher, A., Friedrich, T., Hellwig, P., Nasiri, H.R., Melin F., ‘Identification and optimization of quinolone-based inhibitors against cytochrome bd oxidase using an electrochemical assay’ (2021) Electrochim. Acta 381, 138293.
Sarr, B., Mbaye, A., Diop, C. A. K., Sidibé, M., Melin, F., Hellwig, P., Maury, F., Charvillat, C., Giorgi, M., Michaud, F. Desapt, R. ‘Two new inorganic-organic hybrid materials based on β- and γ-octamolybdate clusters: Synthesis, structure determination and solid-state photochromic properties' (2021), Polyhedron, 194, 114919.
Huo, Z., Liang, Y., Lv, Y., Melin, F., Hellwig, P., Ibrahim, H., Goldmann, M., Boudon, C., Badets, V., Bonnefont, A., Ruhlmann, L. ‘Enhancement of photocurrent by incorporation of Preyssler type polyoxometalate protected nanoparticles in polyporphyrin films’ (2021) Chem. Commun., 57, 1482-1485.
2020
Omeis, F., Santos Seica, A.F., Ermolova, N., R. Kaback H., Hellwig, P. ‘Monoclonal antibody 4B1 influences the pKa of Glu325 in Lactose permease (LacY) from Escherichia coli: evidence from SEIRAS' (2020) FEBS Lett., 594, 3356-3362.
Santos Seiça, A.F., Iancu, C.V., Pfeilschifter, B., Madej, M.G., Choe, J.Y., Hellwig, P. ‘Asp22 drives the protonation state of the Staphylococcus epidermidis glucose/H+ symporter' (2020), J. Biol. Chem., 295, 15253-15261.
Melin, F., Hellwig, P. ‘Redox Properties of the Membrane Proteins from the Respiratory Chain' (2020), Chem. Rev., 120, 10244–10297.
Garcia, A.M.; Giorgiutti, C.; El Khoury, Y.; Bauer, V.; Spiegelhalter, C.; Leize-Wagner, E.; Hellwig, P.; Potier, N.; Torbeev, V. ‘Aggregation and amyloidogenicity of nuclear coactivator binding domain of CREB-binding protein' (2020), Chem. Eur. J., 26, 9889-9890.
Nikolaev, A., Makarchuk, I., Thesseling, A., Hoeser, J., Friedrich, T., Melin, F., Hellwig, P. ‘Stabilization of the Highly Hydrophobic Membrane Protein, Cytochrome bd Oxidase, on Metallic Surfaces for Direct Electrochemical Studies' (2020), Molecules, 25, 3240.
Omeis, F., Santos Seica, A.F., Bernard, R., Javahiraly, N., Majjad, H., Moss, D., Hellwig, P. ‘Following the Chemical Immobilization of Membrane Proteins on Plasmonic Nanoantennas Using Infrared Spectroscopy' (2020), ACS sensors, 5, 2191-2197.
Grytsyk, N.; Boubegtiten-Fezoua, Z.; Javahiraly, N.; Omeis, F.; Devaux, E.; Hellwig P. ‘Surface-enhanced resonance Raman spectroscopy of heme proteins on a gold grid electrode’ (2020), Spectrochim. Acta A, 230, 118081.
Falcone, E.; Ahmed, I.M.M.; Oliveri, V., Bellia, F.; Vileno, B.; El Khoury, Y.; Hellwig, P.; Faller, P.; Vecchio, G. ‘Acrolein and Copper as Competitive Effectors of α‐Synuclein’ (2020), Chem. Eur. J., 26, 1871-1879.
Santos Seica, A.F.; Schimpf, J.; Friedrich, T.; Hellwig, P. ‘Visualizing the movement of the amphipathic helix in the respiratory complex I using a nitrile infrared probe and SEIRAS’ (2020), FEBS Lett., 594, 491-496.
2019
Safarian, S.; Hahn, A.; Mills, D.J.; Radloff, M.; Eisinger, M.L.; Nikolaev, A.; Meier-Credo, J.; Melin, F.; Miyoshi, H., Gennis, R.B.; Sakamoto, J.; Langer, J.D.; Hellwig, P.; Kühlbrandt, W.; Michel, H. ‘Active site rearrangement and structural divergence in prokaryotic respiratory oxidases’ (2019), Science, 366, 100-104.
El Khoury, Y.; Collongues, N.; De Sèze, J.; Gulsari, V.; Patte-Mensah, C.; Marcou, G.; Varnek, A.; Mensah-Nyagan, A.G.; Hellwig, P. ‘Serum-based differentiation between multiple sclerosis and amyotrophic lateral sclerosis by Random Forest classification of FTIR spectra’. (2019), Analyst, 144, 4647-4652.
Gonzalez, P.; Bossak-Ahmad, K.; Vileno, B.; Wezynfeld, N.E.; El Khoury, Y.; Hellwig, P.; Hureau, C.; Bal, W.; Faller, P. ‘Triggering Cu-coordination change in Cu(ii)-Ala-His-His by external ligands.’ (2019), Chem. Commun., 55, 8110-8113.
Mezic, K.G.; Juarez, O., Neehaul, Y.; Cho, J.; Cook, D.; Hellwig, P.; Barquera, B. 'Glutamate-95 in NqrE is an essential residue for the translocation of cations in Na+-NQR' (2019), Biochemistry, 58, 2167-2175.
Sarr, B.; Mbaye, A.; Diop, C. A. K.; Sidibe, M.; Melin, F.; Hellwig, P.; Maury, F.; Senocq, F.; Guionneau, P.; Giorgi, M.; Gautier, R. 'One pot-synthesis of the fourth category of dinuclear molybdenum(VI) oxalate series: Structure and study of thermal and redox properties' (2019) Inorg. Chim. Acta, 491, 84-92.
Grytsyk, N.; Santos Seiça, A.F.; Sugihara, J.; Kaback, H.R.; Hellwig, P. 'Arg302 governs the pKa of Glu325 in LacY' (2019), Proc. Natl. Acad. Sci. U.S.A.,116, 4934-4939.
Gerer, G.; Melin, F.; Hellwig, P.; Hosseini, M. W.; Ferlay, S. 'Chemical and electrochemical alkali cations intercalation/release in an ionic hydrogen bonded network' (2019) Inorg. Chem., 58, 1541-1547.
Patrahau, B.; Chaumont, C.; Barloy, L., Hellwig, P.; Henry, M.; Melin, F.; Pauly, M.; Mobian, P. 'From a bulk solid to thin films of a hybrid material derived from the [Ti10O12(cat)8(py)8] oxo cluster and poly (4-vinylpyridine)' (2019), New. J. Chem., 43, 1581-1588.
2018
Sarr, B.; Mbaye, A.;, Diop, C.A.K.; Melin, F.; Hellwig, P.; Sidibé, M.; Rousselin, Y. 'Crystal structure of bis-(diiso-propyl-ammonium) molybdate' (2018) Acta Cryst., E74, 1682-1685.
Ferlay, S.; Hellwig P; Hosseini, M. W. 'Partially Reversible Thermal-Induced Oxidation During a Dehydration Process in an H-bonded Supramolecular System' (2018) Chemphyschem 19, 3219-3225.
Melin, F.; Sabuncu, S.; Choi, S.K.; Leprince, A.; Gennis, R.B.; Hellwig, P. 'Role of the tightly bound quinone for the oxygen reaction of cytochrome bo3 oxidase from Escherichia coli' (2018) FEBS Lett. 592, 3380-3387.
Mons, C.; Botzanowski, T.; Nikolaev, A.; Hellwig, P.; Cianférani, S.; Lescop, E.; Bouton, C.; Golinelli-Cohen, M.-P. 'The H2O2-Resistant Fe-S Redox Switch MitoNEET Acts as a pH Sensor To Repair Stress-Damaged Fe-S Protein' (2018) Biochemistry, 57, 5616-5628.
Sarr, B.; Diop, C. A. K.; Melin, F.; Sidibe, M.; Hellwig, P.; Michaud, F.; Maury, F.; Senocq, F.; Mbaye, A.; Rousselin, Y. 'Non-hydrothermal synthesis and structure determination of two new beta-octamolybdate (VI) stabilized with dialkylammonium counterions' (2018) J. Mol. Struct., 1170, 44-50.
Heidrich, J.; Junglas, B.; Grytsyk, N.; Hellmann, N.; Rusitzka, K.; Gebauer, W.; Markl, J.; Hellwig, P.; Schneider, D. 'Mg2+ binding triggers rearrangement of the IM30 ring structure, resulting in augmented exposure of hydrophobic surfaces competent for membrane binding' (2018) J. Biol. Chem., 293, 8230-8241.
Senthil Kumar, K.; Šalitroš, I.; Boubegtiten-Fezoua, Z.; Moldovan, S.; Hellwig, P.; Ruben, M. A 'Spin crossover (SCO) active graphene-iron(ii) complex hybrid material' (2018) Dalton Trans., 47, 35-40.
2017
Melin, F.; Nikolaev, A.; Hellwig, P. 'Redox Activity of Cytochromes from the Respiratory Chain' in "Reference Module in Chemistry, Molecular Sciences and Chemical Engineering", Elsevier, 2017.
Gonzalez, P.; Vileno, B.; Bossak, K.; El Khoury, Y.; Hellwig, P.; Bal, W.; Hureau, C.; Faller, P. 'Cu(II) Binding to the Peptide Ala-His-His, a Chimera of the Canonical Cu(II)-Binding Motifs Xxx-His and Xxx-Zzz-His' (2017) Inorg Chem., 56, 14870-14879.
Vermeer, L. S.; Hamon, L.; Schirer, A.; Schoup, M.; Cosette, J.; Majdoul, S.; Pastré, D.; Stockholm, D.; Holic, N.; Hellwig, P.; Galy, A.; Fenard, D.; Bechinger, B. 'Vectofusin-1, a potent peptidic enhancer of viral gene transfer forms pH-dependent α-helical nanofibrils, concentrating viral particles' (2017) Acta. Biomater., 64, 259-268.
Neehaul, Y.; Kriegel, S.; Barquera, B.; Hellwig, P. 'Functional Studies on Membrane Proteins by Means of H/D Exchange in Infrared: Structural Changes in Na+ NQR from V. cholerae in the Presence of Lipids' (2017) in "Methods in Molecular Biology", 1635, 247-257.
Srour, B.; Bruechert, S.; Andrade, S.L.A., Hellwig, P. 'Secondary Structure Determination by Means of ATR-FTIR Spectroscopy' (2017) in "Methods in Molecular Biology", 1635, 195-203.
El Khoury, Y.; Hellwig, P. 'Far infrared spectroscopy of hydrogen bonding collective motions in complex molecular systems' (2017) Chem. Commun., 53, 8389-8399.
Melin, F.; Schoepp-Cothenet, B.; Noor, M. H.; Abdulkarim, S.; Soulimane, T.; Hellwig, P. 'Electrochemical study of an electron shuttle diheme protein: the cytochrome c550 from T. thermophilus' (2017) Inorg. Chim. Acta, 468, 252-259.
Vorburger, P.; Lo, M.; Choua, S.; Bernard, M.; Melin, F.; Oueslati, N.; Boudon, C.; Elhabiri, M.; Wytko, J. A.; Hellwig, P.; Weiss, J. 'A Question of Flexibility in Cytochrome c Oxidase Models' (2017) Inorg. Chim. Acta, 468, 232-238.
Cote, Y.; Nominé, Y.; Ramirez, J.; Hellwig, P.; Stote, R. H. 'Peptide-Protein binding investigated by Far-IR spectroscopy and Molecular Dynamics simulations' (2017) Biophys. J., 112, 2575-2588.
Khalil, M.; Bernhardt, R.; Hellwig, P. 'Raman and infrared spectroscopic evidence for the structural changes of the 2Fe2S cluster and its environment during the interaction of adrenodoxin and adrenodoxin reductase' (2017) Spectrochim. Acta A, 183, 298-305.
, N.; , , H. R.; Hellwig, P. 'pKa of Glu325 in LacY' (2017) Proc. Natl. Acad. Sci. U.S.A., 114, 1530-1535.
2016
Ruiz, J.; Boehringer, R.; Grogg, M.; Raya, J.; Schirer, A.; Crucifix, C.; Hellwig, P.; Schultz P.; Torbeev, V. 'Covalent tethering and residues with bulky hydrophobic side chains enable to direct self-assembly of the distinct amyloid structures' (2016) ChemBioChem, 17, 2274-2285.
Melin, F.; Xie, H.; Meyer, T.; Anh, Y. O.; Gennis, R. B.; Michel, H.; Hellwig, P. 'The unusual redox properties of C-type oxidases' (2016) Biochim. Biophys. Acta, 1857, 1892-1899.
Kao, W.C.; Kleinschroth, T.; Nitschke, W.; Baymann, F.; Neehaul, Y.; Hellwig, P.; Richers, S.; Vonck, J.; Bott, M.; Hunte, C. 'The obligate respiratory supercomplex from Actinobacteria.' (2016) Biochim. Biophys. Acta, 1857, 1705-1714.
Khalil, M.; Boubegtiten-Fezoua, Z.; Hellmann, N., Hellwig, P. 'Extraordinary stability of hemocyanins from L. polyphemus and E. californicum studied using infrared spectroscopy from 294 to 20 K ' (2016) Phys. Chem. Chem. Phys., 18, 28732-28739.
Fournier, E.; Nikolaev, A.; Nasiri, H.; Hoeser, J.; Friedrich, T.; Hellwig, P.; Melin, F. 'Creation of a gold nanoparticle based electrochemical assay for the detection of inhibitors of bacterial cytochrome bd oxidases' (2016) Bioelectrochemistry, 111, 109-114.
Srour, B.; Erhard, B.; Süss, R.; Hellwig, P. 'Monitoring the pH Triggered Collapse of Liposomes in the Far IR Hydrogen Bonding Continuum' (2016) J. Phys. Chem. B., 120, 4047-4052.
Torbeev, V.; Grogg, M.; Ruiz, J.; Boehringer, R.; Schirer, A.; Hellwig, P.; Jeschke, G.; Hilvert, D. 'Chiral recognition in amyloid fiber growth' (2016) J. Pept. Sci., 22, 290-304.
Hellwig, P.; Kriegel, S., Friedrich, T. 'Infrared spectroscopic studies on reaction induced conformational changes in the NADH ubiquinone oxidoreductase (complex I)' (2016) Biochim. Biophys. Acta, 1857, 922-927.
Voicescu, M.; Hellwig, P.; Meghea, A. 'Antioxidant activity of phytoestrogen type isoflavones in biomimetic environments' (2016) New J. Chem., 40, 606-612.
2015
Melin, F.; Hellwig, P. ‘Combining Electrochemistry and Infrared Spectroscopy for the Study of Proteins' in "Reference Module in Chemistry, Molecular Sciences and Chemical Engineering", Elsevier, 2015.
Kriegel, S.; Srour, B.; Steimle, S.; Friedrich, T.; Hellwig, P. ‘Involvement of acidic amino acid residues in Zn2+ binding to respiratory complex I’ (2015) Chembiochem, 16, 2080-2085.
Weinert, T.; Huwiler, S.G.; Kung, J.W.; Weidenweber, S.; Hellwig, P.; Stärk, H.J.; Biskup, T.; Weber, S.; Cotelesage, J.J.; George, G.N.; Ermler, U.; Boll, M. ‘Structural basis of enzymatic benzene ring reduction’ (2015) Nat. Chem. Biol., 11, 586-591.
Shalabney, A.; George, J.; Hiura, H.; Hutchison, J.A.; Genet, C.; Hellwig, P.; Ebbesen T.W. ‘Enhanced Raman Scattering from Vibro-Polariton Hybrid States’ (2015) Angew. Chem. Int. Ed., 54, 7971-7975.
Gaillard, T.; Trivella, A.; Stote, R.H.; Hellwig, P. ‘Far infrared spectra of solid state l-serine, l-threonine, l-cysteine, and l-methionine in different protonation states’ (2015) Spectrochim. Acta A Mol. Biomol. Spectrosc., 150, 301-307.
Paulus, B.; Bajzath, C.; Melin, F.; Heidinger, L.; Kromm, V.; Herkersdorf, C.; Benz, U.,; Mann, L.; Stehle, P.; Hellwig P.; Weber, S.; Schleicher E. 'Spectroscopic Characterization of Radicals and Radical Pairs in Fruit Fly Cryptochrome: Protonated and Non-Protonated Flavin Radical-States’ (2015) FEBS J., 282, 3175-3178.
Meyer, T.; Melin, F.; Richter, O.-M.H.; Ludwig, B.; Kannt, A.; Müller, H.; Michel, H.; Hellwig, P. 'Electrochemistry suggests proton access from the exit site to the binuclear center in Paracoccus denitrificans cytochrome c oxidase pathway variants' (2015) Febs. Lett., 589, 565-568.
Hellwig, P. ‘Infrared spectroscopic markers of quinones in proteins from the respiratory chain’ (2014) Biochim. Biophys. Acta, 1847, 126-133.
2014
Kriegel, S.; Uchida, T.; Osawa, M.; Friedrich, T.; Hellwig, P. 'Biomimetic Environment to Study E. coli Complex I through Surface-Enhanced IR Absorption Spectroscopy' (2014) Biochemistry, 53, 6340-6347.
Shulte, M.; Mattay, D.; Kriegel, S.; Hellwig, P.; Friedrich, T. 'Inhibition of Escherichia coli Respiratory Complex I by Zn2+'(2014) Biochemistry, 53, 6332-6339.
Strickland, M.; Juarez, O.; Neehaul, Y.; Cook, D. A.; Barquera, B.; Hellwig, P. ‘The Conformational Changes Induced by Ubiquinone Binding in the Na+-pumping NADH:Ubiquinone Oxidoreductase (Na+-NQR) Are Kinetically Controlled by Conserved Glycines 140 and 141 of the NqrB Subunit’ (2014) J. Biol. Chem., 289, 23723-23733.
Melin, F.; Noor, M. R.; Pardieu, E.; Boulmedais, F.; Banhart, F.; Cecchini, G.; Soulimane, T.; Hellwig, P. ‘Investigating the Thermostability of Succinate: Quinone Oxidoreductase Enzymes by Direct Electrochemistry at SWNTs-Modified Electrodes and FTIR Spectroscopy’ (2014) Chemphyschem, 15, 3572-3579.
Meyer, T.; Melin, F.; Xie, H.; von der Hocht, I.; Choi, S. K.; Noor, M. R.; Michel, H.; Gennis, R. B.: Soulimane, T.; Hellwig, P. ‘Evidence for distinct electron transfer processes in terminal oxidases from different origin by means of protein film voltammetry’ (2014) J. Am. Chem. Soc., 136, 10854-10857.
2013
Hielscher, R.; Hellwig, P, ‘Specific far infrared spectroscopic properties of phospholipids’ (2013) In “Spectroscopy of biological Molecules” Editors. Marques, M. P; Batista de Carvalho, L. A. E.; Haris, P. I., IOS Press, 2013.
Hielscher, R.; Yegres, M.; Voicescu, M., Gnandt; E., Friedrich; T., Hellwig, P., ‘Characterization of two quinone radicals in the NADH:ubiquinone oxidoreductase from Escherichia coli by a combined fluorescence spectroscopic and electrochemical approach’ (2013) Biochemistry, 52, 8993-9000.
Ihiawakrim, D.; Ovidiu, E.; Melin, F.; Hellwig, P.; Janowska, I.; Begin, D.; Baaziz, W.; Begin-Colin, S.; Pham-Huu, C.; Baati, R., ‘A single-stage functionalization and exfoliation method for the production of graphene in water: stepwise construction of 2D-nanostructured composites with iron-oxide nanoparticles’ (2013) Nanoscale, 5, 9073-9080.
Neehaul, Y.; Juarez, O.; Barquera, B.; Hellwig, P., ‘Infrared Spectroscopic Evidence of a Redox-Dependent Conformational Change Involving Ion Binding Residue NqrB-D397 in the Na+-Pumping NADH:Quinone Oxidoreductase from Vibrio cholerae’ (2013) Biochemistry, 52, 3085-3093.
Melin, F.; Hellwig, P., ‘Recent Advances in the Electrochemistry and Spectroelectrochemistry of membrane proteins’ (2013) Biol. Chem., 394, 593-609.
Melin, F.; Meyer, T.; Lankiang, S.; Choi, S.K.; Gennis, R. B.; Blanck, C.; Schmutz, M.; Hellwig, P., ‘Direct Electrochemistry of Cytochrome bo3 Oxidase at a series of Gold Nanoparticles-Modified Electrodes’ (2013) Electrochem. Commun., 26, 105-108.
2012
Juarez, O.; Neehaul, Y.; Turk, E.; Chahboun, N.; DeMico, J.; Hellwig, P.; Barquera, B., ‘The role of glycine residues 140 and 141 of subunit B in the functional ubiquinone binding site of the Na+ pumping NADH:quinone oxidoreductase from Vibrio chlolerae' (2012) J. Biol. Chem., 287, 25678-25685.
Hielscher, R.; Hellwig, P, ’Specific far infrared spectroscopic properties of phospholipids’ (2012) Spectrosc-Int. J., 27, 525-532.
Neehaul, Y.; Chen, Y.; Werner, C.; Fee, J. A., Ludwig, B.; Hellwig, P., ‘Electrochemical and infrared spectroscopic analysis of the interaction of the Cu(A) domain and cytochrome c(552) from Thermus thermophilus.’ (2012) Biochim. Biophys. Acta, 1817, 1950-1954.
Neehaul, Y.; Juarez, O.; Barquera, B.; Hellwig, P., ‘Thermodynamic contribution to the regulation of electron transfer in the Na+ pumping NADH:quinone oxidoreductase from Vibrio cholerae’ (2012) Biochemistry, 51, 4072-4077.
Voicescu, M.; Hellwig, P., 'Tyrosines in biological electron transfer', in "Tyrosine and Aspartic Acid: Properties, Sources and Healths Benefit", Editors Jones, J. E.; Morano, D. M.., Nova Science Publishers, 2012.
Friedrich, T.; Hellwig, P.; Einsle O., ‘On the mechanism of the respiratory complex I’, in "A structural perspective on complex I", Ed. L. Sazanov, Springer 2012.
Melin, F.; Trivella, A.; Lo, M.; Ruzie, C.; Hijazi, I.; Oueslati, N.; Wytko, J. A.; Boitrel, B.; Boudon, C.; Hellwig, P.; Weiss, J., ‘Comparative studies in series of cytochrome c oxidase models’ (2012) J. Inorg. Biochem., 108, 196-202.
2011
El Khoury, Y.; Dorlet, P.; Faller, P.; Hellwig, P., ‘New Insights into the Coordination of Cu(II) by the Amyloid-B 16 Peptide from Fourier Transform IR Spectroscopy and Isotopic Labeling’ (2011) J. Phys. Chem. B, 115, 14812-14821.
El Khoury, Y.; Hellwig, P., ‘A combined far infrared spectroscopic and electrochemical approach for the study of iron−sulfur proteins’ (2011) Chemphyschem, 12, 2669-2674.
Meyer, T.; Gross, J.; Blanck, C.; Schmutz, M.; Ludwig, B.; Hellwig, P.; Melin, F., ‘Electrochemistry of Cytochrome c(1), Cytochrome c(552), and Cu(A) from the Respiratory Chain of Thermus thermophilus Immobilized on Gold Nanoparticles.’ (2011) J. Phys. Chem. B, 115, 7165-7170.
Lee, D.W.; El Khoury, Y.; Francia, F.; Zambelli, B.; Ciurli, S.; Venturoli, G.; Hellwig, P.; Daldal, F., ‘Zinc Inhibition of Bacterial Cytochrome bc(1) Reveals the Role of Cytochrome b E295 in Proton Release at the Q(o) Site’ (2011) Biochemistry, 50, 4263-4272
El Khoury, Y.; Hielscher, R.; Voicescu, M.; Gross, J.; Hellwig, P., ‘On the specificity of the amide VI band for the secondary structure of proteins’ (2011), Vibr. Spec. 55, 256-266.
Voicescu, M.; Rother D.; Bardischewsky, F.; Friedrich, C.; Hellwig, P., 'A combined fluorescence spectroscopic and electrochemical approach for the study of thioredoxins.' (2011) Biochemistry, 50, 17-24.
Beneyton, B.; Harrak, A.E.; Griffiths, A.D.; Hellwig, P.; Taly, V., ‘Immobilisation of CotA, an extremophilic laccase from Bacillus subtilis on glassy carbon electrodes for biofuel cell applications’ (2011) Electrochem. Commun., 13, 24-27.
MacMillan, F.; Kacprzak, S.; Hellwig, P.; Grimaldi, S.; Michel, H.; Kaupp, M., ‘Elucidating mechanisms in haem copper oxidases: The high-affinity QH binding site in quinol oxidase as studied by DONUTHYSCORE spectroscopy and density functional theory’ (2011) Faraday Discuss. 148, 315-344.
Hielscher, R.; Hellwig, P., ‘Far and mid infrared spectroscopic analysis on the substrate induced structural dynamics of the respiratory complex I’ (2011) Chemphyschem, 12, 217-224.
2010
El Khoury, Y.; Trivella, A.; Hellwig, P., ‘The hydrogen bonding signature of peptides and proteins in the far infrared’ (2010), Terahertz Science and Technology, Vol 3 No 4.
Hellwig, P.; Melin, F., ‘Recent Applications of Infrared Spectroscopy and Microscopy in Chemistry, Biology and Medicine’ in Handbook of Porphyrin Science Editors. Kadish, K. M.; Smith, K. M. ; Guilard, R. Eds.; World Scientific, 2010; Vol 7, Chapter 36, pp 437-493.
Pohl, T.; Spatzal, T.; Aksoyoglu, M.; Schleicher, E.; Rostas, A.M.; Lay, L.; Glessner, U.; Boudon, C.; Hellwig, P.; Weber, S.; Friedrich, T. ‘Spin labeling of the Escherichia coli NADH ubiquinone oxidoreductase (complex I)’ (2010) Biochim. Biophys. Acta, 1797, 1894-1900.
Pawlik, G.; Kulajta, C.; Sachelaru, I.; Schröder, S.; Waidner, B.; Hellwig, P.; Daldal, F.; Koch, H. G., ‘The putative assembly factor CcoH is stably associated with the cbb3-type cytochrome oxidase’ (2010) J. Bacteriol., 192, 6378-6384.
El Khoury, Y.; Trivella, A.; Gross, J.; Hellwig, P., ‘Probing the Hydrogen Bonding Structure in the Rieske Protein’ (2010) Chemphyschem, 11, 3313-3319.
Dreher, C.; Hielscher, R.; Prodöhl, A.; Hellwig, P.; Schneider, D., ‘Characterization of two cytochrome b6 proteins from the cyanobacterium Gloeobacter violaceus PCC 7421’ (2010) J. Bioenerg. Biomemb., 42, 517-526.
Friedrich, T.; Hellwig, P., ‘Redox-induced conformational changes within the Escherichia coli NADH ubiquinoneoxidoreductase (complex I): An analysis by mutagenesis and FT-IR spectroscopy’ (2010) Biochim. Biophys. Acta, 1797, 659-663.
Trivella, A.; Gaillard, T.; Stote, R. H.; Hellwig, P., 'Far infrared spectra of solid state aliphatic amino acids in different protonation states' (2010) J. Chem. Phys., 132, 115105.
Trivella, A.; El Khoury, Y.; Gaillard, T., Y.; Stote, R. H.; Merino, N.; Blanco, F. J.; Hellwig, P., 'Temperature Dependence of the Far Infrared Signature of Internal Hydrogen Bonds in Proteins as Probed for Integrins' (2010) A.I.P. Conf. Proc., 1214, 3-6.
Zanier, K.; Ruhlmann, C.; Melin, F.; Masson, M.; Ould M’hamed ould Sidi, A.; Bernard, X.; Fischer, B.; Brino, L.; Ristriani, T.; Rybin, V.; Baltzinger, M.; Vande Pol, S.; Hellwig, P.; Schultz, P.; Travé, G., 'E6 proteins from diverse Papillomaviruses self associate both in vitro and in vivo' (2010) J. Mol. Biol., 396, 90-104.
Hielscher, R.; Hellwig, P., 'The temperature dependent hydrogen bonding signature of lipids monitored in the far infrared domain' (2010) Chemphyschem, 11, 435-41.
2009
Voicescu, M.; El Khoury, Y.; Martel, D.; Heinrich, M;. Hellwig, P., 'Spectroscopic Analysis of Tyrosine Derivatives: On the Role of the Tyrosine - Histidine Covalent Linkage in Cytochrome c Oxidase' (2009) J. Phys. Chem. B, 113, 13429-36.
Wenz, T.; Hielscher, R.; Hellwig, P.; Schägger, H.; Richers, S.; Hunte, C., 'Role of phospholipids in respiratory cytochrome bc1 complex catalysis and supercomplex formation' (2009) Biochim. Biophys. Acta, 1787, 609-616.
Hielscher, R.; Wenz, T.; Hunte, C.; Hellwig, P., 'Monitoring the redox and protonation dependent contributions of cardiolipin in electrochemically induced FTIR difference spectra of the cytochrome bc1 complex from yeast' (2009) Biochim. Biophys. Acta, 1787, 617-625.
Voicescu, M.; Heinrich, M.; Hellwig, P.; 'Steady-State and Time Resolved Fluorescence Analysis on Tyrosine-Histidine Model Compounds' (2009) J. of Fluorescence, 19, 257-66.
El Khoury, Y.; Hellwig, P., 'Infrared Spectroscopic Characterization of Copper-Polyhistidine from 1800-50 cm-1: Model Systems for the Cu Coordination' (2009) J. Biol. Inorg. Chem., 14, 23-34.
2008
Kohlstädt, M.; Dörner, K.; Labatzke, R.; Koc, C.; Hielscher, R.; Schiltz, E.; Einsle, O.; Hellwig, P.; Friedrich, T., 'Heterologous Production, Isolation, Characterization and Crystallization of a Soluble Fragment of the NADH:Ubiquinone Oxidoreductase (Complex I) from Aquifex aeolicus' (2008) Biochemistry, 47, 13036-13045.
Janzon, J.; Yuan, Q.; Malatesta, F.; Hellwig, P.; Ludwig, B.; Durham, B.; Millett, F., 'Probing the Paracoccus denitrificans cytochrome c1 / c552 interaction by mutagenesis and fast kinetics' (2008) Biochemistry, 47, 12974-12984.
Dürr, K.; Koepke, J.; Hellwig, P.; Müller, H.; Angerer, H.; Peng, G.; Olkhova, E.; Richter, O.-M.H.; Ludwig, B.; Michel, H., 'A D-pathway mutation decouples the Paracoccus denitrificans cytochrome c oxidase by altering the side chain orientation of a distant, conserved glutamate' (2008) J. Mol. Biol,. 384, 865-77.
Friedrich, C.G.; Quentmeier, A.; Bardishewsky, F.; Rother, D.; Orawski, G.; Hellwig, P.; Fischer, J., 'Redox control of chemotrophic sulphur oxidation of Paracoccus pantotrophus' in 'Microbial Sulfur Metabolism' Editors C. Dahl and C.G. Friedrich, Springer Verlag 2008, Chapter 12, page 139- 150.
Kleinschroth, T.; Anderka, O.; Ritter, M.; Stocker, A.; Link, T.A.; Ludwig, B.; Hellwig, P., 'Characterization of mutations in crucial residues around the Qo binding site of the cytochrome bc1 complex from Paracoccus denitrificans' (2008) FEBS J., 275, 4773-4785.
Pohl, T.; Schneider, D.; Hielscher, R.; Stolpe, S.; Dörner, K.; Kohlstädt, M.; Böttcher, B.; Hellwig, P.; Friedrich, T., 'Nucleotide-induced conformational changes in the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)' (2008) Biochem. Soc. Trans., 36, 971-5.
Dreher, C.; Prodöhl, A.; Hielscher, R.; Hellwig, P.; Schneider, D., 'Multiple Step Assembly of the transmembrane Cytochrome b(6)' (2008) J. Mol. Biol., 382, 1057-65.
Dörr, S.; Schade, U.; Hellwig, P. 'Far infrared spectroscopy on hemoproteins: A model compound study from 1800-100 cm-1' (2008) Vibr. Spec., 47, 59-65.
Hellwig, P.; Böhm, A.; Pfitzner, U.; Mäntele, W.; Ludwig, B., 'Spectroscopic study on the communication between a heme a3 propionate, Asp399 and the binuclear center of cytochrome c oxidase from Paracoccus denitrificans' (2008) Biochim. Biophys. Acta, 1777, 220-6.
2007
Dörr, S.; Schade, U.; Hellwig, P.; Ortolani, M., 'Characterisation of the temperature dependent Fe-imidazol vibrational modes in far infrared' (2007) J. Phys. Chem., 111, 14418-22.
Prodohl, A.; Dreher, C.; Hielscher, R.; Hellwig, P.; Schneider, D., 'Heterologous expression and in vitro assembly of the transmembrane cytochrome b6' (2007) Protein Expr. Purif., 56, 279-285.
Quentmeier, A.; Janning, P.; Hellwig, P.; Friedrich C.G., 'Activation of the heterodimeric central complex SoxYZ of chemotrophic sulfur oxidation is linked to a conformational change and SoxY-Y interprotein disulfide formation' (2007) Biochemistry, 46, 10990-10998.
Forzi, L.; Hellwig, P.; Thauer, R.K.; Sawers, R.G., 'The CO and CN- ligands to the active site Fe in [NiFe]-hydrogenase of Escherichia coli have different metabolic origins' (2007) FEBS Lett., 581, 3317-3321.
Reijerse, E.J.; Sommerhalter, M.; Hellwig, P., Quentmeier, A.; Rother, D.; Laurich, C.; Bothe, E.; Lubitz, W.; Friedrich, C.G., 'The Unusal Redox Centers of SoxXA, a Novel c-Type Heme-Enzyme Essential for Chemotrophic Sulfur-Oxidation of Paracoccus pantotrophus' (2007) Biochemistry, 46, 7804-7810.
Yang, K.; Zhang, J.; Vakkosoglu, A.; Hielscher, R.; Osborne, J.R.; Hemp, J.; Miyoshi, H.; Hellwig, P.; Gennis, R.B., ‘Glutamate 107 in subunit I of the cytochrome bd quinol oxidase from Escherichia coli is protonated and is near the heme d/heme b595 binuclear center’ (2007) Biochemistry, 46, 3270-3278.
Wenz, T.; Covian, R.; Hellwig, P.; MacMillan, F.; Meunier, B.; Trumpower, B.L.; Hunte, C., ‘Mutational analysis of cytrochrome b at the ubiquinol oxidation site of yeast complex III' (2007) J. Biol. Chem., 282, 3977-3988.
2006
Li, W.W.; Hellwig, P.; Ritter, M.; Hähnel, W. ‘De novo design, synthesis and characterization of quinoproteins’ (2006) Chem. Eur. J., 12, 7236-7245.
Maklashina, E.; Hellwig, P.; Rothery, R.A.; Kotlyar, V., Sher, Y.; Weiner, J.H.; Cecchini, G., ’Differences in protonation of ubiquinone and menaquinone in fumarate reductase from Escherichia coli’(2006) J. Biol. Chem., 281, 26655-26664.
Flemming, D.; Hellwig, P.; Lepper, S.; Kloer, D.P.; Friedrich, T., ‘Catalytic importance of acidic amino acids on subunit NuoB of the Escherichia coli NADH: Ubiquinone oxidoreductase (complex I)’(2006) J. Biol. Chem., 281, 24781-24789.
Wenz, T.; Hellwig, P.; MacMillan, F.; Meunier,B.; Hunte, C., ‘Probing the role of E272 in quinol oxidation of mitochondrial complex III’ (2006) Biochemistry, 45, 9042-9052.
Wolpert, M.; Hellwig, P., ‘Infrared spectra and molar absorption coefficients of the 20 alpha amino acids in aqueous solutions in the spectral range from 1800 to 500 cm-1’ (2006) Spectrochim. Acta A, 64, 987-1001.
Dörr, S.; Wolpert, M.; Hellwig, P., ‘Study on the redox state dependent γ(CH) vibrational modes of the c-type heme’(2006) Biopolymers, 82, 349-352.
Hielscher, R.; Wenz, T.; Stolpe, S.; Hunte, C.; Friedrich, T.; Hellwig, P., ‘Monitoring redox-dependent contribution of lipids in Fourier transform infrared difference spectra of complex I from Escherichia coli’ (2006) Biopolymers, 82, 291-294.
2005
Flemming, D.; Stolpe, S.; Schneider, D.; Hellwig, P.; Friedrich, T., ‘A possible role for iron-sulfur cluster N2 in proton translocation by the NADH: Ubiquinone oxidoreductase (Complex I)’ (2005) J. Mol. Microbiol. Biotechn. 10, 208-222.
Friedrich, T.; Stolpe, S.; Schneider, D.; Barquera, B.; Hellwig, P., ‘Ion translocation by the Escherichia coli NADH:ubiquinone oxidoreductase (complex I)’ (2005), Biochem. Soc. Trans. 33,836-839 Part 4.
Bardischewsky, F.; Quentmeier, A.; Rother, D.; Hellwig, P.; Kostka, S.; Friedrich, C.G., ‘Sulfur dehydrogenase of Paracoccus pantotrophus: the molybdoproteindiheme cytochrome c-complex is active without the heme c2 domain’ (2005) Biochemistry 44, 7024-7034.
Richter, O.M.; Dürr, K.L.; Kannt, A.; Ludwig, B.; Scandurra, F.M.; Giuffre, A.; Sarti, P.; Hellwig, P., ‘Probing the access of protons to the K-pathway in the Paracoccus denitrificans cytochrome c oxidase’ (2005) Eur. J. Biochem, FEBS J. 272, 404-12.
2004
Zhang, J.; Hellwig, P.; Osborne, J.P.; Gennis, R.B., ‘Arginine-391 in subunit I of the cytochrome bd quinol oxidasefrom E. coli stabilizes the reduced form of heme b558 and is essential for quinol oxidase activity’ (2004) J. Biol. Chem., 279, 53980-53987.
Quentmeier, A.; Hellwig, P.; Bardischewsky, F.; Friedrich, C.G., ‘Sulfide dehydrogenase activity of the monomeric flavoprotein SoxF of Paracoccus pantotrophus’ (2004) Biochemistry, 43, 14696-703.
Ritter, M.; Palsdottir, H.; Abe, M.; Mäntele, W.; Hunte, C.; Miyoshi, H.; Hellwig P., ‘Direct evidence for the interaction of stigmatellin with an acidic group in the bc1 complex from S.cerevisiae as monitored by FTIR difference spectroscopy and specific 13C labeling’ (2004) Biochemistry, 43, 8439-8446.
Wolpert, M.; Maneg, O.; Ludwig, B.; Hellwig, P., ‘Electrochemical and FT-IR spectroscopic characterization of the CuA center in the cytochrome c oxidase from T. thermophilus for the spectral range from 1800-500 cm-1’ (2004) Biopolymers, 74, 73-76.
Hellwig, P.; Stolpe, S.; Friedrich, T., ‘FTIR spectroscopic study on the conformational reorganisation in E. coli complex I due to redox-driven proton translocation’ (2004) Biopolymers, 74, 69-72.
2003
Quentmeier, A.; Hellwig, P.; Bardischewsky, F.; Grelle, G.; Kraft, R.; Friedrich C.G., ‘Sulfur oxidation in Paracoccus pantotrophus: Interaction of the sulfur-binding protein Sox YZ with the dimanganese SoxB protein’ (2003) Biochem. Biophys. Res. Commun., 312, 1011-1018.
Ritter, M.; Anderka, O.; Ludwig, B.; Mäntele, W.; Hellwig, P., ‘Electrochemical and FTIR spectroscopic characterization ofthe bc1 complex from P. Denitrificans’ (2003) Biochemistry, 42, 12391-12399.
Hellwig, P.; Gomes, C.; Teixeira, M., ‘FTIR spectroscopic characterization of the cytochrome aa3 from Acidianus ambivalens: evidence for the involvement of acidic residues in redox coupled proton translocation.’ (2003) Biochemistry, 42, 6179-6184.
Flemming, D.; Hellwig, P.; Friedrich, T., ‘Involvement of tyrosines 114 and 139 of subunit NuoB in the proton pathway around cluster N2 in E. coli NADH:ubiquinone oxidoreductase’ (2003) J. Biol. Chem. 278,3055-3062.
2002
Hellwig, P.; Soulimane, T.; Mäntele, W., ‘Electrochemical, FTIR and UV/Vis spectroscopic properties of the caa3 oxidase from T. Thermophilus’ (2002) Eur. J. Biochem., 269, 4830-4838.
Hellwig, P.; Yano, T.; Ohnishi, T.; Gennis, R.B., ‘Identification of the residues involved in stabilization of the semiquinone radical in the high affinity UQ binding site in cytochrome bo3 from E. coli by site-directed mutagenesis and EPR spectroscopy’ (2002) Biochemistry, 41, 10675-10679.
Hellwig, P.; Pfitzner, U.; Behr, J.; Rost, B.; Pessavento, R. P.; von Donk, W.; Gennis, R.B.; Michel, H.; Ludwig, B.; Mäntele,W., ‘Vibrational modes of tyrosines in cytochrome c oxidase from P.denitrificans: FTIR and electrochemical studies on Tyr-D4-labeled and on Tyr280His and Tyr35Phe mutant enzymes’ (2002) Biochemistry, 41, 9116-9125.
Zhang, J.; Gennis, R.B.; Oettmeier, W.; Hellwig, P., ‘FTIR spectroscopic evidence for the involvement of an acidic residue inquinone binding in cytochrome bd from E. coli’ (2002) Biochemistry, 41, 4612-4617.
Barquera, B.; Hellwig, P.; Zhou, W.; Morgan, J.E.; Häse, C.C.; Gosink, K.K.; Nilges, M.; Bruesehoff, P.; Roth, A.; Lancaster, C.R.D.; Gennis, R.B., ‘Purification and Characterization of the Recombinant Na+ - Translocating NADH: Quinone Oxidoreductase From Vibrio cholerae’ (2002) Biochemistry 41, 3781-3789.
2001
Zhang, J.; Hellwig, P.; Osborne, J.P.; Huang, H.W.; Moenne-Loccoz, P.; Konstantinov, A.A.; Gennis, R.B., ‘Site-directed mutagenesis at the highly conserved region near the Q-loop of cytochrome bd quinol oxidase in Escherichia coli specifically perturbs heme b595’(2001) Biochemistry, 40, 8548-8556.
Hellwig, P.; Rost, B.; Mäntele, W., ‘Redox dependent conformational changes in the mixed valence form of the cytochrome coxidase from P. denitrificans: The reorganization of glutamic acid 278 is coupled to the electron transfer from/to heme a and the binuclear center’ (2001) Spectrochim. Acta A, 57, 1123-1131.
Hellwig, P.; Barquera, B.; Gennis, R.B.,‘Direct Evidence for the Protonation of Aspartate-75, Proposed to be at a Quinol Binding Site, upon Reduction of Cytochrome bo3 from E. coli’ (2001) Biochemistry, 40,1077-82.
2000
Friedrich, T.; Brors, B.; Hellwig, P.; Kintscher, L.; Rasmussen, T.; Scheide, D.; Schulte, U.; Mäntele, W.; Weiss, H., ‘Characterization of two novel redox groups in the respiratory NADH:ubiquinone oxidoreductase (complex I)’ (2000) Biochim. Biophys. Acta., 1459, 305-309.
Behr, J.; Michel, H.; Mäntele, W.; Hellwig P., ‘Functional properties of the heme propionates in cytochrome c oxidase from Paracoccus denitrificans. Evidence from FTIR difference spectroscopy and site-directed mutagenesis’ (2000) Biochemistry, 39, 1356-1363.
Hellwig, P.; Scheide, D.; Bungert, S.; Mäntele, W.; Friedrich, T., ‘FT-IR spectroscopic characterization of NADH:ubiquinone oxidoreductase (complex I) from Escherichia coli: oxidation of FeS cluster N2 is coupled with the protonation of an aspartate or glutamate side chain’ (2000) Biochemistry, 39, 10884-10891.
1999
Kuglstatter, A.; Hellwig, P.; Fritzsch, G.; Wachtveitl, J.; Oesterhelt, D.; Mäntele, W.; Michel, H., ‘Identification of a hydrogen bond in the Phe M197 -> Tyr mutant reaction center of the photosynthetic purple bacterium Rhodobacter sphaeroides by X-ray crystallography and FTIR spectroscopy.’ (1999) FEBS Lett., 463,169-74.
Hellwig, P.; Mogi, T.; Tomson, F.L.; Gennis, R.B.; Iwata, J.; Miyoshi, H.; Mäntele, W., ‘Vibrational modes of ubiquinone in cytochrome bo3 from Escherichia coli identified by Fourier Transform difference spectroscopy and specific 13C labeling’(1999) Biochemistry, 38, 14683-14689.
Hellwig, P.; Soulimane, T.; Buse, G.; Mäntele, W., ‘Similarities and dissimilarities in the structure-function relationbetween the cytochrome c oxidase from bovine heart and from Paracoccus denitrificans as revealed by FT-IR spectroscopy’ (1999) FEBS Lett., 458, 83-86.
Kannt, A.; Pfitzner, U.; Ruitenberg, M.; Hellwig, P.; Ludwig, B.; Mäntele, W.; Fendler K.; Michel, H., ‘Mutation of Arg-54 strongly influences heme composition and rate and directionality of electron transfer in Paracoccus denitrificans cytochrome c oxidase’ (1999) J. Biol. Chem., 274, 37974-81.
Hellwig, P.; Soulimane, T.; Buse, G.; Mäntele, W., ‘Electrochemical, FT-IR and UV/VIS spectroscopic properties of the ba3 oxidase from T. Thermophilus’ (1999) Biochemistry, 38, 9648-9658.
Rost, B.; Behr, J.; Richter, O.-M.H.; Hellwig, P.; Ludwig, B.; Michel, H.; Mäntele, W., ‘Time-resolved FT-IR studies on the CO adduct of Paracoccus denitrificans cytochrome c oxidase: comparison of the fully reduced and the mixed valence form’ (1999) Biochemistry, 38, 7565-71.
Hellwig, P.; Grzybek, S.; Behr, J.; Ludwig, B.; Michel, H.; Mäntele, W., ‘Electrochemical and UV/VIS/IR-Spectroscopic Analysis of Heme a and a3 Redox Reactions in the Cytochrome c Oxidase from Paracoccus denitrificans: Separation of Heme a and a3 contributions and assignments of vibrational modes’ (1999) Biochemistry, 38, 1685-1694.
1998
Hellwig, P.; Ostermeier, C.; Ludwig, B.; Michel, H.; Mäntele, W., ‘Electrochemically induced FT-IR difference spectra of the two- and four-subunit cytochrome c oxidase from P. denitrificans reveal identical conformational changes upon redox transitions’ (1998) Biochim. Biophys. Acta., 1409, 107-112.
Behr, J.; Hellwig, P.; Mäntele, W.; Michel, H.,‘Redox dependent changes at the heme propionates in the cytochrome coxidase from Paracoccus denitrificans: direct evidence from FTIR difference spectra and heme propionate 13C labelling’ (1998) Biochemistry, 37, 7400-7406.
Hellwig, P.; Behr, J.; Ostermeier, C.; Richter,O.-M.H.; Pfitzner, U.; Odenwald, A.; Ludwig, B., Michel, H.; Mäntele, W.,‘Involvement of glutamic acid 278 in the redox reaction of the cytochrome c oxidase from Paracoccus denitrificans investigated by FT-IRspectroscopy’ (1998) Biochemistry, 37,7390-7399.