Publications


2017

"Omics for Precious Rare Biosamples: Characterization of Ancient Human Hair by a Proteomic Approach"

M. Fresnais, P. Richardin, M. Sepulveda, E. Leize-Wagner, A. Charrié-Duhaut, OMICS: a Journal of Integrative Biology, 2017, 21(7), 361-370. (pdf)


"Top-down and middle-down approach by fraction collection enrichment using Off-line CE-UV/MALDI-MS: application to monoclonal antibody Fc/2 charge variants"

M. Biacchi, N. Said, A. Beck, E. Leize-Wagner, YN. François, Journal of Chromatography A, 2017, 1498, 120-127. (pdf)

Audioslide presentation


"Advanced antibody-drug conjugate structural characterization by sheathless capillary electrophoresis-tandem mass spectrometry using complementary approaches"

N. Said, J. Giorgetti, L. Kuhn, A. Beck, E. Leize-Wagner, R. Gahoual, YN. François, LCGC North America, 2017, 15, 15-21 (pdf)


 


2016

"Cutting-edge capillary electrophoresis characterization of monoclonal antibodies and related products"

R. Gahoual, A. Beck, E. Leize-Wagner, YN. François, Journal of Chromatography B, 2016, 1032, 61-78 (pdf)


"Structural characterization of antibody drug conjugate by a combination of intact, middle-up and bottom-up techniques using sheathless capillary electrophoresis – tandem mass spectrometry as nanoESI infusion platform and separation method"

N. Said, R. Gahoual, L. Kuhn, A. Beck, YN. François, E. Leize-Wagner, Analytica Chimica Acta, 2016, 28, 50-59 (pdf)

Audioslide presentation


"Iminosugar-Cyclopeptoid Conjugates Raise Multivalent Effect in Glycosidase Inhibition at Unprecedented High Level"

ML. Lepage, JP. Schneider, A. Bodlenner, A. Meli, F. De Riccardis,  M. Schmitt, C. Tarnus, NT. Nguyen-Huynh, YN. Francois, E. Leize-Wagner, C. Birck, A. Cousido-Siah, A. Podjarny, I. Izzo, P. Compain, European-A European Journal, 2016, 22, 5151-5155 (pdf)


"Characterization of Cetuximab Fc/2 subunits aggregates by Off-line CZE-MS"

Y.-N. Francois, M. Biacchi, N. Said, C. Renard, A. Beck, R. Gahoual, E. Leize-Wagner, Analytica Chimica Acta, 2016, 908, 168-176 (pdf)

Audioslide presentation

 

"Improvement of mitochondria extract from Saccharomyces cerevisiae characterization in shotgun proteomic using sheathless capillary electrophoresis coupled to tandem mass spectrometry"

M. Ibrahim, R. Gahoual, L. Enkler, H. Becker, J. Chicher, P. Hammann, Y.-N. Francois, L. Kuhn, E. Leize-Wagner, Journal of Chromatographic Science, 2016, 54(4), 653-663 (pdf)

 

"Monitoring of the Retinoic Acid Receptor - Retinoid X Receptor dimerization upon DNA binding by native mass spectrometry"

N.-T. Nguyen-Huynh, J. Osz, C. Peluso-Iltis, N. Rochel, N. Potier, E. Leize-Wagner, Biophysical Chemistry, 2016, 210, 2-8

 

"Innovative Characterization of Hair from pre-Hispanic Andean Mummies using Mass spectrometry""

M. Fresnais, P. Richardin, M. Sepulveda, E. Leize-Wagner, A. Charrié-Duhaut, Proceedings of the 64th ASMS Conference on Mass Spectrometry and Allied Topics, 2016.

 

"Glycoform Separation and Characterization of Cetuximab Variants by Middle-up Off-line CE-UV/ESI-MS"

N. Said, M. Biacchi, R. Gahoual, C. Renard, A. Beck, Y.-N. Francois, E. Leize-Wagner, Proceedings of the 64th ASMS Conference on Mass Spectrometry and Allied Topics, 2016.

 

"High-order Structural Interrogation of Antibody-drug Conjugates by a Combination of Intact, Middle-up and Bottom-up techniques using Sheathless Capillary Electrophoresis-Msas Spectrometry"

N. Said, R. Gahoual, L. Kuhn, A. Beck, Y.-N. Francois, E. Leize-Wagner, Proceedings of the 64th ASMS Conference on Mass Spectrometry and Allied Topics, 2016.

 

"La spectrométrie de masse, une technique de choix pour l'analyse moléculaire de baumes de momies"

A. Charrié-Duhaut, J. Hertzog, Spectra Analyse, 2016, 312, 54-60

 

"L'apport de l'analyse protéomique à l'étude des cheveux de momies"

Margaux Fresnais, A. Charrié-Duhaut, Spectra Analyse, 2016, 312, 61-69


"Caractérisation fine de protéines thérapeutiques à l’aide du couplage de l’électrophorèse capillaire et de la spectrométrie de masse"

R. Gahoual, M. Biacchi, N. Said, A. Beck, E. Leize-Wagner, YN. Francois, Spectra Analyse, 2016, 311, 52-59 (pdf)



2015

"Independent highly sensivite characterization of asparagine deamidation and aspartic acid isomerization by sheathless CZE-ESI-MS/MS"

R. Gahoual, A. Beck, Y.-N. Francois, E. Leize-Wagner, Journal of Mass Spectrometry, 2015, 51(2), 150-158 (pdf)

 

"Comprehensive Multilevel Characterization of Biologics Usinig Sheathless Capillary Electrophoresis Hyphenated to Tandem Mass Spectrometry"

R. Gahoual, M. Biacchi, JM. Busnel, A. Beck, Y-N. Francois, E. Leize-Wagner, LCGC North America, 2015, 13 (4), 8-15 (pdf)


"Priming the Cells for Their Final Destination: Microenvironment Controlled Cell Culture by a modular ECM-mimicking Feeder Film" (pdf)

J. Barthes, E. Vrana, H. Ozcelik, R. Gahoual, Y. Francois, J. Bacharouche, G. Francius, J. Hemmerlé, M-H. Metz-Boutigue, P. Schaaf, P. Lavalle, Biomaterials Science, 2015, 3, 1302-1311

 

"Glycoform Separation and Characterization of Cetuximab Variants by Middle-up Off-Line Capillary Zone Electrophoresis-UV/Electrospray Ionisation-MS"

M. Biacchi, R. Gahoual, N. Said, A. Beck, E. Leize-Wagner, Y.-N. Francois, Analytical Chemistry, 2015, 87, 6240-6250 (pdf)


"Chemical cross-linking and mass spectrometry to determine the subunit interaction network in a recombinant juman SAGA HAT subcomplex"

N.-T. Nguyen-Huynh, G. Sharov, C. Potel, P. Fichter, S. Trowitzsch, I. Berger, V. Lamour, P. Schultz, N. Potier, E. Leize-Wagner, Protein Science, 2015, 8, 1232-1246

 

"Recent advances in the characterization of hair of mummies from the Chilean Andean coast"

M. Fresnais, P. Richardin, A. Gimat, M. Sepulveda, E. Leize-Wagner, A. Charrié, Forensic Science International, 2015, 249, 25-34

 

"Chemical cross-linking and mass spectrometry to determine the interation network of protein complexes"

N.-T. Nguyen-Huynh, G. Sharov, C. Potel, P. Fichter, S. Trowitzsch, I. Berger, V. Lamour, P. Schultz, N. Potier, E. Leize-Wagne, Proceedings of the 63rd ASMS Conference on Mass Spectrometry and Allied Topics, 2015.


2014

"O12: Analysis of Chilean mummies hair: Recent advance in their characterization"

M. Fresnais, P. Richardin, A. Charrié, M. Sepulveda, E. Leize-Wagner, Toxicologie Analytique et Clinique, Proceedings of the Analytical, Clinical and Forensic Toxicology International Meeting, 2014, 26, S9-S10


"Novel sheathless CE-MS interface as an original and powerful infusion platform for nanoESI study: from intact proteins to high molecular mass noncovalent complexes"

R. Gahoual, J-M. Busnel, P. Wolff, Y-N. François, E. Leize-Wagner, Analytical and Bioanalytical Chemistry, 2014, 406, 1029-1038 (pdf)

 

“Full antibody primary structure and micro-variant characterization in a single injection using transient isotachophoresis and sheath-less capillary electrophoresis – tandem mass spectrometry”

R. Gahoual, J-M. Busnel, A. Beck, Y-N. François, E. Leize-Wagner, Analytical Chemistry, 2014, 86 (18):9074-9081 (pdf)

 

"Globin's Structure and Function in Vesicomyid Bivalves from the Gulf of Guinea Cold Seeps as an Adaptation to Life in Reduced Sediments"

C. Decker, N. Zorn, N. Potier, E. Leize-Wagner, F.H. Lallier, K. Olu, A.C. Andersen, Physiological and Biochemical Zoologyl, 2014, 87 (6):855-869

 

« Analysis of monoclonal antibody by a novel CE-UV/MALDI-MS interface »

M. Biacchi, R. Bhajun, N. Saïd, Y-N. François, E.Leize-Wagner, Electrophoresis, 2014, 20, 2986-2995 (pdf)

 

“Monoclonal antibodies biosimilarity assessment using transient isotachophoresis capillary zone electrophoresis-tandem mass spectrometry”

R. Gahoual, M. Biacchi, J. Chicher, L. Kuhn, P. Hammann, A. Beck, E. Leize-Wagner, Y-N. François, mAbs, 2014, 6 (6), 1464-1473 (pdf)

 

"Intact protein separation by CE-UV/MALDI-MS for top-down proteomics"

M. Biacchi, A. Beck, Y-N. Francois, E. Leize-Wagner, Proceedings of the 62th ASMS Conference on Mass Spectrometry and Allied Topics, 2014.

 

"Monoclonal antibodies complete primary structure and biosimilarity assessment in a single analysis using transient isotachophoresis sheathless capillary electrophoresis-tandem mass spectrometry"

R. Gahoual, J-M. Busnel, J. Chicher, L. Kuhn, P. Hammann, A. Beck, Y-N. Francois, E. Leize-Wagner, Proceedings of the 62th ASMS Conference on Mass Spectrometry and Allied Topics, 2014.


2013

"Structural insight into negative DNA supercoiling by DNA gyrase, a bacterial type 2A DNA topoisomerase"

J. Papillon, J-F. Ménétret, C. Batisse, R. Hélye, P. Schultz, N. Potier, V.Lamour, Nucleic Acids Research, 2013, vol. 41, issue 16, p.7815-7827


"Rapid and multi-level characterization of trastuzumab using sheathless capillary electrophoresis-tandem mass spectrometry"

R. Gahoual,A. Burr, J-M. Busnel, L. Kuhn, P. Hammann, A. Beck, Y-N. François, E. Leize-Wagner, mAbs, 2013, vol. 5, p.479-490 (pdf)

 

"Novel family members of CYP109 from Sorangium cellulosum So ce56 exhibit characteristic biochemical and biophysical properties"

Y. Khatri, F. Hannemann, M. Girhard, R. Kappl, A. Même, M. Ringle, S. Janoche, E. Leize-Wagner, V.B. Urlacher, R. Bernhardt, Biothechnology and Applied Biochemistry, 2013, vol.60, issue 1, p.18-29

 

"The asymmetric binding of PGC-1a to the ERRa and ERRy nuclear receptor homodimers involves a similar recognition mechanism"

M. Takacs, M.V. Petovkhov, R.A. Atkinson, P. Roblin, F-X. Ogi, B. Demeler, N. Potier, Y. Chebaro, A. Dejaegere, D.I. Svergun, I.M.L. Billas, PLOS One, 2013, vol. 8, issue 7, p.1-11

 

"Molecular evidence of bitumen in the Mousterian lithic assemblage of Hummal (Central Syria)"

T.C. Hauck, J. Connan, A. Charrié-Duhaut, J-M. Le Tensorer, H. al Sakhel,  Journal of Archaeological Science, 2013, vol. 40, issue 8, 3252-3262

 

"First molecular identification of a hafting adhesive in the Late Howiesons Poort at Diepkloof Rock Shelter (Western Cape, South Africa)"

A. Charrié-Duhaut, G. Porraz, C.R. Cartwright, M. Igreja, J. Connan, C. Poggenpoel, P-J. Texier, Journal of Archaeological Science, 2013, vol.40, issue 9, 3506-3518

 

"The MSA sequence of Diepkloof and the history of southern African Late Pleistocene populations" 

G. Porraz, J.E. Parkington, J-P. Rigaud, C.E. Miller, C. Poggenpoel, C. Tribolo, W. Archer, C.R. Cartwright, A. Charrié-Duhaut, L. Dayet, M. Igreja, N. Mercier, P. Schmidt, C. Verna, P-J. Texier, Journal of Archaeological Science, 2013, vol. 40, issue 9, 3542-3552

 

"Simultaneous peptide mapping, posttranslational modifications and major N-glycosylation characterization of trastuzumab by sheathless CE-ESI-MS/MS - Comparison to nanoLC-MS/MS"

R. Gahoual, A. Burr, J-M. Busnel, L. Kuhn, P. Hammann, A. Beck, Y-N. François, E. Leize-Wagner, Proceedings of the 61th ASMS Conference on Mass Spectrometry and Allied Topics, 2013.

 

"CE-UV/MALDI-MS: a new platform for proteomic and intact protein characterization"

M. Biacchi, A. Resemann, P-O. Schmit, A. Beck,Y-N. François, E. Leize-Wagner, Proceedings of the 61th ASMS Conference on Mass Spectrometry and Allied Topics, 2013.


2012

"A novel MALDI-MS approach for the analysis of neutral metallosupramolecular architectures"

A. Même, A.R. Stefankiewicz, J. Harrowfield, X-Y. Cao, J. Huuskonen, K. Rissanen, J-M. Lehn, H. Nierengarten, E. Leize, European Journal of Inorganic Chemistry, 2012, 4, 647-654

 

"Biomarqueurs et GC/MS: des principes clés pour la caractérisation, la conservation et la restauration des objets du patrimoine. Applicaton à un adhésif végétal néolithique."

A. Charrié, C. Leprovost, Spectra Analyse, 2012, 825, 32-39

 

"Sheathless Capillary Electrophoresis as an Infusion Platform for the NanoESI Study of Intact Proteins and Non-Covalent Complexes"

R. Gahoual ; Y-N. Francois, E. Leize-Wagner,Proceedings of the 60th ASMS Conference on Mass Spectrometry and Allied Topics, 2012.

 

"Enrichment by fraction collection developed for CE/MALDI-MS to analyze proteins by Top Down strategy"

M. Biacchi, R. Bhajun, Y.N. François, E. Leize-Wagner, Proceedings of the 60th ASMS Conference on Mass Spectrometry and Allied Topics, 2012


2010

"Structural characterization of hemoglobins from Monilifera and Frenulata tube worms (Siboglinids): First discovery of giant hexagonal-bilayer hemoglobin in the former "Pogonophora" group"

C. Meunier, AC Andersen, M. Bruneaux, D. Le Guen, P. Terrier, E. Leize-Wagner, F. Zal, Comp Biochem Physiol A Mol Integr Physiol., 2010, Part A 155, 41-48.



"Insights into metazoan evolution from alvinella pompejana cDNAs"

N. Gagnière, D. Jollivet, I. Boutet, Y. Brévilet, D. Busso, C. Da Silva, F. Gaill, D. Higuet, S. Hourdez, B. Knoops, F. Lallier, E. Leize-Wagner, J. Mary, D. Moras, E. Perrodou, JF. Rees, B. Segurens, B. Shillito, A. Tanguy, JC. Thierry, J. Weissenbach, P. Wincker, F. Zal,O. Poch, O. Lecompte, BMC Genomics, 2010, 11, 634.



"The "Phantom Effect" of the rexinoid LG100754: structural and functional insights"

Y. Sato, N. Ramalanjanoa, T. Huet, N. Potier, J. Osz, P. Antony, C. Peluso-Iltis, P. Poussin-Courtemagne, E. Ennifar, Y. Mély, A. Dejaere, D. Moras, N. Rochel, PLoS One (2010) Nov 30 :5(11) :e15119


2009

"Mass spectrometry contribution for stability constants measurement of protein/protein complexes"

A. Même, P. Terrier, F. Hannemann, R. Bernhardt, H. Nierengarten, N. Potier, E. Leize-Wagner, Proceedings of the 57th ASMS Conference on Mass Spectrometry and Allied Topics, 2009.



"Power and limitations of electrophoretic separations in proteomics strategies"

T. Rabilloud, AR. Vaezzadeh, N. Potier, C. Lelong, E. Leize-Wagner, M. Chevallet, Mass Spectrom Rev., 2009, 28, 816-43.



"Structural study of Carcinus maenas hemocyanin by native ESI-MS: interaction with L-lactate and divalent cations"

M. Bruneaux, P. Terrier, E. Leize, J. Mary, FH. Lallier, F. Zal, Proteins, 2009, 77, 589-601.



"A novel generic and effective method for the rapid purification of G-protein-coupled receptors"

T. Magnin, C. Fiez-Vandal, N. Potier, A. Coquard, I. Leray, T. Steffan, C. Logez, F. Alkhalfioui, F. Pattus, R. Wagner, Protein Exp. Purif., 2009, 64, 1-7.



"Crystal growth of proteins, nucleic acids and viruses in gels"

B. Lorber, C. Sauter, A. Théobald-Dietrich, A. Moreno, P. Schellenberger, MC. Robert, B. Capelle, S. Sanglier, N. Potier, R. Giegé, Prog Biophys Mol Biol. 2009, 101 (1-3), 13-25


2008

"Aldosterone: from biosynthesis to non-genomic action on the proteome"

S. Zöllner, KH. Hwang, B. Wilzewski, C. Carapito, E. Leize-Wagner, A. Van Dorsselaer, R. Bernhardt, Steroids, 2008, 73, 966-72.



"Respiratory adaptations of a deep-sea hydrothermal crustacean: The case of Segonzacia mesatlantica"

M. Bruneaux, J. Pasquier, P. Terrier, E. Leize, F. Lallier, F. Zal, Comparativ Biochemistry and Physiology - Part A: Molecular& Integrative Physiology, 150, 2008, S119.


2007

"Ruthenium(II) complexes with improved photophysical properties based on planar 4'-(2-Pyrimidinyl)-2,2' :6,2''-terpyridine ligands"

Y.Q. Fang, N.J. Taylors, F. Laverdiere, G.S. Hanan, F. Loiseau, F. Nastasi, S. Campagna, H. Nierengarten, E. Leize-Wagner, A. Van Dorsselaer, Inorg. Chem., 2007, 46 2854-2863.



"A tale of two oxidation states : Bacterial colonization of arsenic-rich environments"

D. Muller, c. Medigue, S. Koechler, V. Barbe, M. Barakat, E. Talle, V. Bonnefroy, E. Krin, F. Arsene-Ploetze, C. Carapito, M. Chandler, B. Cournoyer, S. Cruveiller, C. Dossat, M. Heymann, E. Leize, A. Lieutaud, D. Lievremont, Y. Makita, S. Mangenot, W. Nitschke, P. Ortet, N. Perdrail, B. Schoepp, P. Siguier, DD. Simeonova, Z. Rouy, B. Segurens, E. Turlin, D. Vallenet, A. Van Dorsselaer, S. Weiss, J. Weissenbach, MC. Lett, A. Danchin, PN. Bertin, Plos Genetics, 2007, 3.



"Short term adaptive, response and phenotypic plasticity of Crustacean hemocyanins : Study of Carcinus maenas and Segonzacia mesatlantica"

M. Bruneaux, F. Zal, E. Leize, Comparative Biochemistry and Physiology A-Molecular & Integrative Physiology, 2007, 146, S85-S86



"Macrotricycles featuring a pi-basic tetrahedral cavity : preference for NH4+ detected by ESI-MS"

A. Lelias-Vanderperre, JC. Chambron, E. Espinosa, P. Terrier, E. Leize-Wagner, Organic Letters, 2007, 9, 2961-2964.



"The structural analysis of large noncovalent oxygen binding proteins by MALLS and ESI-MS: previous works and new insights concerning annelid hexagonal bilayer hemoglobin and crustacean hemocyanin"

M. Bruneaux, M. Rousselot, E. Leize, FH. Lallier, F. Zal, Current Protein and Peptide Science, 2007, 2008, 9, 15-80.



"Supramolecular circular helicates formed by destabilisation of supramolecular dimers"

J. Hamblin, F. Tuna, S. Bunce, LJ Childs, A. Jackson, W. Errington, NM. Alcock, H. Nierengarten, A. Van Dorsselaer, E. Leize-Wagner, MJ Hannon, Chemistry, 2007, 13, 9286-96.



"Lanthanide complexation with CMPO and CMPO-calix[4]arenes in solution: spectrophotometric and electrospray mass spectrometric approaches"

H. Herschbach, F. Brisach, J. Haddaoui, M. Saadioui, E. Leize, A. Van Dorsselaer, F. Arnaud-Neu, V. Böhmer, Talanta, 2007, 74, 39-46.



"Determination of aqueous inclusion complexation constants ans stoichiometry of alkyl(methyl)-methylimidazolium-based ionic liquid cations and neutral cyclodextrins by affinity capillary electrophoresis"

Y. François, A. Varenne, J. Sirieix-Plenet, P. Gareil, Journal of Separation Science, 2007, Volulme 30, Issues 5, Pages 751-760. (pdf)



"Nonaqueous capillary electrophoretic behavior of 2-aryl propionic acids in the presence of an achiral ionic liquid : A chemometric approach"

Y. François, A. Varenne, E. Juillerat, A-C. Servais, P. Chiap, P. Gareil, Journal of Chromatography A, 1138, (1-2), 2007, Pages 268-275. (pdf)



"Evaluaton of chiral ionic liquids as additives to cyclodextrins for enantiomeric separations by capillary electrophoresis"

Y. François, A. Varenne, E. Juillerat, D. Villemin, P. Gareil, Journal of Chromatography A, Volume 1155, Issue 2, 2007, Pages 134-141. (pdf)


2006

"Alterations of the mitochondrial proteome caused by the absence of mitochondrial DNA : a proteomic view"

M. Chevalet, P. Lescuyer, H. Diemer, A. Van Dorsselaer, E. Leize-Wagner, T. Rabilloud, Electrophoresis, 2006, 27, 1574-1583.



"Preparation of alumina supported gold catalysts : gold complexes genesis, identification and speciation by mass spectrometry"

S. Ivanova, V. Pichon, C. Petit, H. Herschbach, A. Van Dorsselaer, E. Leize, Applied Catalysis, 2006, 298, 203-210.



"Oxidative stess response : a proteomic view"

T. Rabilloud, M. Chevallet, S. Luche, E. Leize-Wagner, Expert Review of Proteomics, 2005, 2, 949-956.



"Analysis of aldosterone-induced differential receptor-independent protein patterns using 2D-electrophoresis and mass spectrometry"

S. Bohmer, C. Carapito, B. Wilzewski, E. Leize, A. Van Dorsselaer, R. Bernhardt, Biol Chem., 2006, 387, 917-929.



"Electrospray mass spectrometry analysis of dendritic branches bearing peripheral fullerene subunits"

H. Herschbach, K. Hosomizu, U. Hahn, E. Leize, A. Van Dorsselaer, H. Imahori, JF. Nierengarten, Anal Bioanal Chem., 2006, 386, 46-51.



"Proteome analysis of Schizosaccharomyces pombe by two-dimensional gel electrophoresis and mass spectrometry"

K. Hwang, C. Carapito, S. Bohmer, E. Leize, A. Van Dorsselaer, R. Bernhardt, Proteomics, 2006, 6, 4115-4129.



"Synthesis of fullerodendrons with an ammonium unit at the focal point and their cooperative self-assembly on a fluorescent ditopic crown ether receptor"

JF. Nierengarten, U. Hahn, A. Trabolsi, H. Herschbach, F. Cardinali, M. Elhabiri, E. Leize; A. Van Dorsselaer, AM. Albrecht-Gary, Chemistry, 2006, 12, 3365-3373.



"Improved mass spectrometry compatibility is afforded by ammoniacal silver staining"

M. Chevalet, H. Diemer, S. Luche; A. Van Dorsselaer, T. Rabilloud, E. Leize-Wagner, Proteomics, 2006, 6, 2350-2354.



"New integrated measurement protocol using capillary electrophoresis instrumentation for the determination of viscosity, conductivity and absorbance of ionic liquid-molecular solvent mixtures"

Y. François, K. Zhang, A. Varenne, P. Gareil, Analytica Chimica Acta, Volume 562, Issue 2, 2006, Pages 164-170. (pdf)